*América Mineiro/MG 1-0 Bahia/BA
| 19.05.2016 | Bahia/BA | 0-1 | América Mineiro/MG | |
| 12.05.2016 | América Mineiro/MG | 0-0 | Bahia/BA | |
*Ypiranga/RS 4-3 Aparecidense/GO
| 20.05.2016 | Aparecidense/GO | 2-1 | Ypiranga/RS | |
| 12.05.2016 | Ypiranga/RS | 3-1 | Aparecidense/GO | |
Dom Bosco/MT 2-7 Atlético/PR *
| 19.05.2016 | Atlético/PR | 5-0 | Dom Bosco/MT | |
| 12.05.2016 | Dom Bosco/MT | 2-2 | Atlético/PR | |
*Bragantino/SP 2-1 Avai FC/SC
| 11.05.2016 | Avai FC/SC | 1-1 | Bragantino/SP | |
| 04.05.2016 | Bragantino/SP | 1-0 | Avai FC/SC | |
River/PI 0-2 Botafogo/PB *
| 19.05.2016 | Botafogo/PB | 1-0 | River/PI | |
| 12.05.2016 | River/PI | 0-1 | Botafogo/PB | |
Joinville/SC 0-2 Ceará SC/CE *
| 18.05.2016 | Ceará SC/CE | 1-0 | Joinville/SC | |
| 12.05.2016 | Joinville/SC | 0-1 | Ceará SC/CE | |
Paraná/PR 2-3 Chapecoense/SC *
| 19.05.2016 | Chapecoense/SC | 2-0 | Paraná/PR | |
| 12.05.2016 | Paraná/PR | 2-1 | Chapecoense/SC | |
*Juventude/RS 3-2 Coritiba/PR
| 20.05.2016 | Coritiba/PR | 2-2 | Juventude/RS | |
| 12.05.2016 | Juventude/RS | 1-0 | Coritiba/PR | |
Ferroviária/SP 3-6 Fluminense/RJ *
| 13.05.2016 | Fluminense/RJ | 3-0 | Ferroviária/SP | |
| 05.05.2016 | Ferroviária/SP | 3-3 | Fluminense/RJ | |
Sampaio Correa/MA 1-3 Figueirense/SC *
| 07.07.2016 | Figueirense/SC | 1-0 | Sampaio Correa/MA | |
| 12.05.2016 | Sampaio Correa/MA | 1-2 | Figueirense/SC | |
*Fortaleza/CE 4-2 Flamengo/RJ
| 19.05.2016 | Flamengo/RJ | 1-2 | Fortaleza/CE | |
| 05.05.2016 | Fortaleza/CE | 2-1 | Flamengo/RJ | |
*Gama/DF 2-2 ABC Natal/RN
| 16.06.2016 | ABC Natal/RN | 1-1/p.1-4 | Gama/DF | |
| 02.06.2016 | Gama/DF | 1-1 | ABC Natal/RN | |
Genus/RO 0-4 Ponte Preta/SP *
| 13.05.2016 | Ponte Preta/SP | 3-0 | Genus/RO | |
| 06.05.2016 | Genus/RO | 0-1 | Ponte Preta/SP | |
Juazeirense/BA 1-3 Botafogo/RJ *
| 20.05.2016 | Botafogo/RJ | 1-0 | Juazeirense/BA | |
| 13.05.2016 | Juazeirense/BA | 1-2 | Botafogo/RJ | |
Londrina/PR 0-2 Cruzeiro/MG *
| 11.05.2016 | Londrina/PR | 0-2 | Cruzeiro/MG | |
Operário F./PR 1-2 Paysandu/PA *
| 07.07.2016 | Paysandu/PA | 2-0 | Operário F./PR | |
| 18.05.2016 | Operário F./PR | 1-0 | Paysandu/PA | |
Portuguesa/SP 1-3 Vitória/BA *
| 20.05.2016 | Vitória/BA | 3-1 | Portuguesa/SP | |
| 12.05.2016 | Portuguesa/SP | 0-0 | Vitória/BA | |
CRB/AL 1-2 Vasco da Gama/RJ *
| 19.05.2016 | Vasco da Gama/RJ | 1-1 | CRB/AL | |
| 12.05.2016 | CRB/AL | 0-1 | Vasco da Gama/RJ | |
Vitoria Conquista/BA 0-2 Santa Cruz/PE *
| 12.05.2016 | Vitoria Conquista/BA | 0-2 | Santa Cruz/PE | |